行为核心服务

正规澳门赌场网络的服务

The 行为的核心 offers a variety of models and measures commonly used in the research setting including general behavioral phenotyping, 神经行为测试, 以及体内药物筛选.

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工作和培训服务的要求是通过实验室提出的.

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If there is a model or measure you are interested in but don’t see here email us at behaviorcore@zhongxinboligang.com. 

A student reviews a microscopic image of neurons on a computer screen

模型

核心提供尖端, validated and well characterized models commonly used in basic science research. These models are used to gain mechanistic insights to clinically relevant questions and for in vivo screening in drug development.

  • 伤害感觉(急性和慢性模型)
  • 学习与记忆
  • 神经认知功能
  • 焦虑和抑郁
  • 上瘾
  • 药代动力学剂量和收集

措施

Assays commonly used as behavioral readouts for basic science research.

诱发/急性疼痛措施

  • 冯弗雷试验(点状触觉过敏)
    A non-noxious punctate tactile stimulus is applied to the desired test area. The threshold to evoke a withdrawal response is quantified.
  • 刷测(动态触觉超敏反应)
    A non-noxious dynamic brushing tactile stimulus is applied to the desired test area. 所引起的反应是分级的.
  • 兰德尔塞利托压力超敏试验
    在测试区域施加钝压力刺激. 引起反应的阈值是量化的.
  • 平彻试验(压力超敏反应)
    在测试区域施加钝压力刺激. 引起反应的阈值是量化的.
  • 压力应用管理(P.A.M.)(关节过敏)
    在测试区域施加钝压力刺激. 引起反应的阈值是量化的.
  • 静态负重(关节过敏)
    测量后肢之间的重量变化.
  • 哈格里夫斯试验(热过敏)
    A heat stimulus is applied to the test area through a glass plate. 唤起戒断反应的潜伏期是量化的.
  • 甩尾试验(热过敏)
    测试区域浸在预热过的浴池中. 唤起戒断反应的潜伏期是量化的.
  • 干冰试验(冷超敏反应)
    通过玻璃板将干冰施加于测试区域. 唤起戒断反应的潜伏期是量化的.
  • 热/冷板(热过敏)
    A hot or cold plate used to measure changes in thermal sensitivity. Subjects are placed on the pre-heated or pre-cooled metal plate and the latency to evoke a response is measured.
  • 握力
    This test can be used to measure forepaw and/or hindpaw muscular strength, 以及对轴向拉伸的过度敏感.

动机/认知措施

  • 有条件的地方偏好(CPP)
    This test is used to measure the motivational effects of reinforcing experiences.
  • 成瘾潜力
    有回报的药物.g. cocaine, heroin) produce conditioned place preference for a novel context 
  • Antinociceptive潜在
    减轻持续疼痛的药物.g. neuropathic pain) produce conditioned place preference for a novel context
  • 条件性场所回避(CPA)
    This is used to measure the motivational effects of aversive experience:  
  • 令人厌恶的药物或经历.g. loud noise, noxious events) produce conditioned place aversion for a novel context.
  • Conflict Avoidance Measure (Coy Mechanical Conflict-Avoidance System)
    Used to assess diminished motivation to cross a textured floor to a preferred dark space. 
  • 热环境偏好(温暖或寒冷)
    This is used to measure inclination to explore a surface that is hot or cold compared to a room temperature surface.
  • 开放田地测试
    This test is used to assess spontaneous locomotor activity and rearing. Time spent in margins versus the center of the open field is used as a measure of anxiety.
  • 措施 to determine antidepressant-like states for assessment of novel antidepressant drugs are available upon request (e.g. 游泳测试).
  • 措施 to determine anxiety-like states for assessment of novel anxiolytic drugs are available upon request (e.g. EPM).
  • 措施 to determine learning and memory are available upon request (e.g. 新目标识别，t型迷宫，莫里斯水迷宫).

额外的措施

  • Rotorod测试
    这是对运动协调和平衡的测试.
  • GI过境(木炭)测试
    This is a measure of the level of gastrointestinal inhibition of opioid-like compounds in drug development screening.
  • 宽容/依赖
    Measure the level of tolerance and/or dependence that develops after chronic use of opioid-like compounds in drug development screening.

手术模型

  • 术后疼痛
    • 足底切口(小鼠/大鼠)
  • 神经性疼痛
    • 坐骨神经压迫(小鼠/大鼠)
    • 残存神经损伤(小鼠/大鼠)
    • 眶下慢性缩窄性损伤(大鼠)
  • 骨关节炎
    • 半月板部分切除术

 

与博士合作. King and her team at the UNE Behavioral Core has been a seamless process [for EydisBio]. I have been impressed with the breath of models and assays offered as well as the experience and professionalism of the team. 利用博士. King’s experience and expertise in pre-clinical pain modeling has greatly enhanced our study designs. Furthermore, the post study data package was clear and well organized.” — Scott Scarneo, Director of 研究 and Development at EydisBio

附加服务

  • We offer handling and compound administration options to assist in all aspects of drug screening, 模型开发和研究实施.
  • The core is accomplished in perfusions and tissue collection methods.
  • Our 工作人员 is trained to preform pharmacokinetic (PK) dosing experiments.
  • We offer consultation services for grant submissions, UNE 机构动物护理和使用委员会 方案提交和研究设计.

培训

We are available to conduct trainings on any of our services or equipment, 对教师, 工作人员, 和学生.

学生 and 工作人员 are welcome to complete UNE’s Rodent Behavior Skills badge to display on their transcript and/or resume. 访问我们的Credly徽章网站了解更多信息

A professor watches over a student as they work with lab equipment
Two students work together to prepare a slide for the microscope

质量控制

Our 工作人员 have more than 17 years of combined experience with behavior testing in a research environment. All experimental procedures will obtain prior approval by the IACUC and will be conducted in compliance with the NIH Guide for the Care and Use of Laboratory Animals.