任务
的 mission of the 组织学 和 Imaging Core is to provide Center for 疼痛 研究 investigators 和 the greater UNE research community access to expertise, Training, 和 specialized instrumentation related to tissue processing, 切片, 染色, 免疫组织化学, 和显微镜.
Additionally the Core offers Service 和 Training related to image analysis 和 image analysis software to guide investigators in choosing the best methods for presenting their data. We also accommodate work for outside facilities on a fee-for-service basis.
的se images represent work completed by 组织学 和 Imaging Core professional staff. All samples were processed on core equipment, 和 images captured on core microscopes. 的 gallery contains a wide variety of tissue types, including nervous tissue, 骨, 海带, 和 drosophila larvae.
Service
UNE COBRE 组织学 和 Imaging Core Fee Structure
| Service | 单位 | 内部费用 | External Academic Fee | External Commerical Fee |
|---|---|---|---|---|
| Miscellaneous labor, Training | 每小时 | $41 | $63 | $126 |
| Decalcification, EDTA or formic acid | 每个样品，周 | $3.62 | $4.92 | $9.85 |
| Paraffin processing, st和ard | 每一盒 | $2.24 | $2.89 | $5.78 |
| Paraffin processing, mega | 每一盒 | $2.80 | $3.45 | $6.91 |
| 嵌入、石蜡 | 每一块 | $2.18 | $3.20 | $6.41 |
| 嵌入、冷冻 | 每一块 | $3.75 | $4.90 | $9.81 |
| Paraffin Microtomy, Routine | 每张幻灯片 | $1.89 | $2.76 | $5.52 |
| Paraffin Microtomy, Levels | 每张幻灯片 | $2.44 | $3.59 | $7.18 |
| Cryo切片, up to 3 sections 每张幻灯片 | 每张幻灯片 | $2.36 | $3.13 | $6.26 |
| Cryo切片, 3 or more sections 每张幻灯片 | 每张幻灯片 | $3.80 | $5.33 | $10.67 |
| H&E染色，手工 | 每张幻灯片 | $2.44 | $3.26 | $6.51 |
| Special Staining, Tier 1 (TB, Picrosirius, PAS, Mucicarmine) | 每张幻灯片 | $3.13 | $4.42 | $8.84 |
| Special Staining, Tier 2 (Masson's Trichrome, Safranin O/Fast Green) | 每张幻灯片 | $4.65 | $6.53 | $13.06 |
| IHC/IF, Paraffin (user-supplied primary antibody) | 每张幻灯片 | $7.67 | $10.64 | $21.27 |
| IHC/I,F Frozen (user-supplied primary antibody) | 每张幻灯片 | $6.08 | $8.45 | $16.91 |
样品处理
的 组织学 和 Imaging Core is fully integrated with the UNE的行为核心 和 can directly process samples collected by the 行为的核心.
Training
的 Core also provides Training to professional staff 和 students on equipment 和 techniques.
的 core has cryostats, microtomes, 和 microscopes available for reservation. To avoid scheduling conflicts 和 maintenance issues, all equipment must be reserved using Outlook calendars, 和 individual users must be trained by core staff.
的 Leica confocal has a separate Training procedure consisting of two 2-hour supervised sessions, several informational videos, 还有一个在线测试. 的 first Training session is an introduction to the microscope guided by core staff. 的 second session entails imaging of a user-generated sample with minimal intervention from Core staff. Upon completion of a proficiency quiz, users can reserve time as desired.
For any Training inquiries, please email 彼得Caradonna at pcaradonna@zhongxinboligang.com.
徽章
的 UNE徽章计划 is a non-credit bearing vehicle that digitally validates 和 documents knowledge, 技能, 资格, 和能力. 对所有学习者开放, any participant who completes the learning activities 和 demonstrates achievement of the learning outcomes/competencies for a badge would be able to claim it. Sharing badges with graduate schools, potential employers, etc., is a great way to demonstrate some of the skills 和能力 you have acquired that are not captured in an academic transcript.
For interested students, the core is now part of the UNE徽章计划. 学生 will gain a knowledge-base 和 skillsets relevant to the core specialty.
资金
Assistance is available for help to cover the fees for work performed by the research cores.
出版物
Karamchedu NP, Fleming BC, Proffen BL, Sant NJ, Portilla G, Parola LR, Molino J, Murray MM. Terminal sterilization influences the efficacy of an extracellular matrix-blood composite for treating posttraumatic osteoarthritis in the rat model. J骨科. 2022 Mar;40(3):573-583.
Eaton VE, Pettit S, Elkinson A, Houseknecht KL, 王TE, May M. Polymicrobial abscess following ovariectomy in a mouse. BMC兽医中心. 2019 Oct 24;15(1):364.
Sannajust S, Imbert I, Eaton V, Henderson T, Liaw L, May M, Barbe MF, 王T. Females have greater susceptibility to develop ongoing pain 和 central sensitization in a rat model of temporom和ibular joint pain. 疼痛. 2019 Sep;160(9):2036-2049.
Gjelsvik K, Follansbee T, Ganter G. Bone Morphogenetic Protein Glass Bottom Boat (BMP5/6/7/8) 和 its receptor Wishful Thinking (BMPRII) are required for injury-induced allodynia in Drosophila. 分子的痛苦. Jan-Dec 2018;14:1744806918802703.
Sherman H, Gitschier HJ, 和 Rossi AE. A Novel Three-Dimensional Immune Oncology Model for High-Throughput Testing of Tumoricidal Activity. 前面. Immunol.2018年4月23日.
Havelin J, Imbert I, Cormier J, Allen J, Porreca F, 王T. Central Sensitization 和 Neuropathic Features of Ongoing 疼痛 in a Rat Model of Advanced Osteoarthritis. J疼痛. 2016 Mar;17(3):374-82.
一个交易, 埃里克森K, 突然的年代, 缅甸人米, Limbic system development underlies the emergence of classical fear conditioning during the 3rd 和 4th weeks of life in the rat. Behavioral Neuroscience. Behav >. 2016 Apr;130(2):212-30.
华纳E, Krivitsky R, 锥K, 阿瑟顿P, Pitre T, Lanpher J, Giuvelis G, Bergquist我, 王T, Bilsky E, 史蒂文森G. Evaluation of a Postoperative 疼痛-Like State n Motivated Behavior in Rats: Effects of Plantar Incision on Progressive-Ratio Food-Maintained Responding. 药物开发研究. Dec 2015; 76(8):432–441.
缅甸M，比尔斯基E. FAAH inhibitor OL-135 disrupts contextual, but not auditory, fear conditioning in rats. 行为与大脑. 2016年7月15日;308:1-5.
缅甸人米A, Simmons CA, Hughes M, Lei L. Developing 和 validating trace fear conditioning protocols in C57BL/6 mice. J神经科学方法. 2014 Jan 30;222:111-7.
Mclane V, Cao L, Willis C. Morphine increases hippocampal viral load 和 suppresses frontal lobe CCL5 expression in the LP-BM5 AIDS model. J Neuroimmunol. 2014 Apr 15;269(1-2):44-51.
Please remember to acknowledge the Center for 疼痛 研究 组织学 和 Imaging Core 和 the financial support provided by the NIGMS grant number P20GM103643 in manuscripts, 奖助金, 和 presentations that include our work.
联系
位置
小卡车219/220
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